T-SPOT.TB检测是一种基于 T 细胞的干γ-干扰素释放试验 （IGRA）的检测方法，旨在获得在广泛患者群中的准确结果，即使在免疫状态较弱的患者中也可实现。T-SPOT.TB 检测将 T 细胞从全血中分离出来，并标准化每个患者测试中这些细胞的数量。T-SPOT.TB 还对细胞进行检测，以去除可能影响检测结果的潜在干扰物质。在 T-SPOT.TB检测设计中包含这些可降低结果错误或不确定的风险步骤。
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